resource source identifier antibodies rabbit polyclonal anti fip200 proteintech  (Proteintech)

Journal: Acta Histochemica et Cytochemica

Article Title: Spatial Transcriptomics Meets Histochemistry: Insights from Glioblastoma as a Model System

doi: 10.1267/ahc.25-00065

Figure Lengend Snippet: Spatial transcriptomics-based in situ hybridization–like expression mapping of the TOP2A-LGALS1-immune microenvironments axis and validation by immunohistochemistry. ( A ) To simulate an in situ hybridization–like pattern, spatial transcriptomic data for TOP2A, LGALS1, CD69, and CD4 were mapped onto the tissue section. TOP2A and LGALS1 expression was markedly upregulated in the tumor region compared with the non-tumorous area located in the lower right of the section. In contrast, no corresponding increase in the expression of CD69 or CD4, which are presumed to be negatively regulated by LGALS1, was observed. Bars = 1 mm. ( B ) Immunohistochemical staining using specific antibodies against TOP2A, LGALS1, CD69, and CD4 demonstrated expression patterns parallel to those observed in panel ( A ), confirming that the mRNA expression profiles identified by spatial transcriptomics are similarly reflected at the protein level. Microvessels are indicated by arrows. Bars = 75 μm.

Article Snippet: Importantly, subsequent immunohistochemical analysis by use of (rabbit polyclonal antibody to TOP2A (Proteintech, 20233-1-AP, at 1:400 dilution) and to Galectin-1 (Proteintech, 11858-1-AP, at 1:1000 dilution) provided additional spatial resolution at the protein level, demonstrating that both TOP2A and LGALS1 showed particularly enhanced expression in tumor cells located in close proximity to microvessels ( B).

Techniques: Spatial Transcriptomics, In Situ Hybridization, Expressing, Biomarker Discovery, Immunohistochemistry, Immunohistochemical staining, Staining